[Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].
Identifieur interne : 003A26 ( Main/Exploration ); précédent : 003A25; suivant : 003A27[Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].
Auteurs : Ping-Ping Lv [République populaire de Chine] ; Jun Hu ; Shao-Liang Chen ; Xin Shen ; Wei-Bo Yin ; Yu-Hong Chen ; Yong-Ru Sun ; Zan-Min HuSource :
- Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology [ 1671-3877 ] ; 2007.
Descripteurs français
- KwdFr :
- Antiport des ions sodium-hydrogène (génétique), Antiport des ions sodium-hydrogène (physiologie), Chlorure de sodium (pharmacologie), Expression des gènes (effets des médicaments et des substances chimiques), Mutation (MeSH), Populus (génétique), Protéines végétales (génétique), Protéines végétales (physiologie), RT-PCR (MeSH), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (physiologie), Test de complémentation (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Expression des gènes, Saccharomyces cerevisiae.
- génétique : Antiport des ions sodium-hydrogène, Populus, Protéines végétales, Saccharomyces cerevisiae.
- pharmacologie : Chlorure de sodium.
- physiologie : Antiport des ions sodium-hydrogène, Protéines végétales, Saccharomyces cerevisiae.
- Mutation, RT-PCR, Test de complémentation.
English descriptors
- KwdEn :
- Gene Expression (drug effects), Genetic Complementation Test (MeSH), Mutation (MeSH), Plant Proteins (genetics), Plant Proteins (physiology), Populus (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (physiology), Sodium Chloride (pharmacology), Sodium-Hydrogen Exchangers (genetics), Sodium-Hydrogen Exchangers (physiology).
- MESH :
- chemical , genetics : Plant Proteins, Sodium-Hydrogen Exchangers.
- drug effects : Gene Expression, Saccharomyces cerevisiae.
- genetics : Populus, Saccharomyces cerevisiae.
- chemical , pharmacology : Sodium Chloride.
- chemical , physiology : Plant Proteins, Saccharomyces cerevisiae, Sodium-Hydrogen Exchangers.
- Genetic Complementation Test, Mutation, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na(+)/H(+) antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.
PubMed: 17452804
Affiliations:
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Le document en format XML
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des médicaments et des substances chimiques)</term><term>Mutation (MeSH)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (physiologie)</term><term>RT-PCR (MeSH)</term><term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (physiologie)</term><term>Test de complémentation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Sodium-Hydrogen Exchangers</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Expression des gènes</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="genetics" 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PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17452804</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>27</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1671-3877</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>2</Issue><PubDate><Year>2007</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology</Title><ISOAbbreviation>Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].</ArticleTitle><Pagination><MedlinePgn>173-8</MedlinePgn></Pagination><Abstract><AbstractText>Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na(+)/H(+) antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Ping-Ping</ForeName><Initials>PP</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shao-Liang</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Wei-Bo</ForeName><Initials>WB</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yu-Hong</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yong-Ru</ForeName><Initials>YR</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Zan-Min</ForeName><Initials>ZM</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao</MedlineTA><NlmUniqueID>101156321</NlmUniqueID><ISSNLinking>1671-3877</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant 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MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012965" MajorTopicYN="N">Sodium Chloride</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017923" MajorTopicYN="N">Sodium-Hydrogen Exchangers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>4</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>4</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17452804</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Shao Liang" sort="Chen, Shao Liang" uniqKey="Chen S" first="Shao-Liang" last="Chen">Shao-Liang Chen</name><name sortKey="Chen, Yu Hong" sort="Chen, Yu Hong" uniqKey="Chen Y" first="Yu-Hong" last="Chen">Yu-Hong Chen</name><name sortKey="Hu, Jun" sort="Hu, Jun" uniqKey="Hu J" first="Jun" last="Hu">Jun Hu</name><name sortKey="Hu, Zan Min" sort="Hu, Zan Min" uniqKey="Hu Z" first="Zan-Min" last="Hu">Zan-Min Hu</name><name sortKey="Shen, Xin" sort="Shen, Xin" uniqKey="Shen X" first="Xin" last="Shen">Xin Shen</name><name sortKey="Sun, Yong Ru" sort="Sun, Yong Ru" uniqKey="Sun Y" first="Yong-Ru" last="Sun">Yong-Ru Sun</name><name sortKey="Yin, Wei Bo" sort="Yin, Wei Bo" uniqKey="Yin W" first="Wei-Bo" last="Yin">Wei-Bo Yin</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lv, Ping Ping" sort="Lv, Ping Ping" uniqKey="Lv P" first="Ping-Ping" last="Lv">Ping-Ping Lv</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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